Identification of 38kDa Brugia malayi microfilarial protease as a vaccine candidate for lymphatic filariasis.
Identifieur interne : 007F78 ( Main/Exploration ); précédent : 007F77; suivant : 007F79Identification of 38kDa Brugia malayi microfilarial protease as a vaccine candidate for lymphatic filariasis.
Auteurs : K N Krithika [Inde] ; Pankaj Dabir ; Sandeep Kulkarni ; V. Anandharaman ; M V R. ReddySource :
- Indian journal of experimental biology [ 0019-5189 ] ; 2005.
Descripteurs français
- KwdFr :
- Agranulocytes (immunologie), Agranulocytes (métabolisme), Animaux, Anticorps antihelminthe (), Anticorps antihelminthe (isolement et purification), Antigènes d'helminthe (), Antigènes d'helminthe (isolement et purification), Brugia malayi (métabolisme), Chromatographie d'affinité, Chromatographie en phase liquide, Cytokines (métabolisme), Facteurs temps, Filariose lymphatique (), Humains, Immunoglobuline G (), Immunotransfert, Interféron gamma (métabolisme), Interleukine-10 (métabolisme), Interleukine-4 (métabolisme), Lymphocytes auxiliaires Th1 (immunologie), Microfilaria (métabolisme), Peptide hydrolases (), Peptide hydrolases (pharmacologie), Système immunitaire, Électrophorèse sur gel de polyacrylamide.
- MESH :
- immunologie : Agranulocytes, Lymphocytes auxiliaires Th1.
- isolement et purification : Anticorps antihelminthe, Antigènes d'helminthe.
- métabolisme : Agranulocytes, Brugia malayi, Cytokines, Interféron gamma, Interleukine-10, Interleukine-4, Microfilaria.
- pharmacologie : Peptide hydrolases.
- Animaux, Anticorps antihelminthe, Antigènes d'helminthe, Chromatographie d'affinité, Chromatographie en phase liquide, Facteurs temps, Filariose lymphatique, Humains, Immunoglobuline G, Immunotransfert, Peptide hydrolases, Système immunitaire, Électrophorèse sur gel de polyacrylamide.
English descriptors
- KwdEn :
- Animals, Antibodies, Helminth (chemistry), Antibodies, Helminth (isolation & purification), Antigens, Helminth (chemistry), Antigens, Helminth (isolation & purification), Brugia malayi (metabolism), Chromatography, Affinity, Chromatography, Liquid, Cytokines (metabolism), Electrophoresis, Polyacrylamide Gel, Elephantiasis, Filarial (prevention & control), Elephantiasis, Filarial (therapy), Humans, Immune System, Immunoblotting, Immunoglobulin G (chemistry), Interferon-gamma (metabolism), Interleukin-10 (metabolism), Interleukin-4 (metabolism), Leukocytes, Mononuclear (immunology), Leukocytes, Mononuclear (metabolism), Microfilariae (metabolism), Peptide Hydrolases (chemistry), Peptide Hydrolases (pharmacology), Th1 Cells (immunology), Time Factors.
- MESH :
- chemical , chemistry : Antibodies, Helminth, Antigens, Helminth, Immunoglobulin G, Peptide Hydrolases.
- chemical , isolation & purification : Antibodies, Helminth, Antigens, Helminth.
- immunology : Leukocytes, Mononuclear, Th1 Cells.
- metabolism : Brugia malayi, Cytokines, Interferon-gamma, Interleukin-10, Interleukin-4, Leukocytes, Mononuclear, Microfilariae.
- chemical , pharmacology : Peptide Hydrolases.
- prevention & control : Elephantiasis, Filarial.
- therapy : Elephantiasis, Filarial.
- Animals, Chromatography, Affinity, Chromatography, Liquid, Electrophoresis, Polyacrylamide Gel, Humans, Immune System, Immunoblotting, Time Factors.
Abstract
A FPLC purified 38kDa protease (Bm mf S-7) isolated from B. malayi microfilarial soluble antigen was identified. It showed pronounced reactivity with sera collected from 'putatively immune' asymptomatic and amicrofilaraemic individuals residing in an endemic area for bancroftian filariasis. Further the immune protective activity of Bm mf S-7 antigen was evaluated in susceptible hosts, jirds (Meriones unguiculatus) against B. malayi filarial infection. The antigen showed 89% cytotoxicity against mf and 87-89% against infective (L3) larvae in in vitro antibody dependent cellular cytotoxicity Assay (ADCC) and in situ micropore chamber methods. Bm mf S-7 immunized jirds after challenge infection showed 81.5% reduction in the adult worm burden. The present study has shown that, the 38kDa microfilarial proteases (Bm mf S-7) could stimulate a strong protective immune response against microfilariae and infective larvae in jird model to block the transmission of filariasis. Analysis of IgG subclasses against Bm mf S-7 revealed a significant increase in IgG2 and IgG3 antibodies in endemic normals. Lymphocyte proliferation to Bm mf S-7 was significantly high in endemic normal group as compared to that in clinical and microfilarial carriers. Significantly enhanced levels of IFN-gamma in the culture supernatant of PBMC of endemic normals followed by stimulation with Bm mf S-7 suggest that the cellular response in this group is skewed towards Th 1 type.
PubMed: 16187525
Affiliations:
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Le document en format XML
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(metabolism)</term><term>Interleukin-10 (metabolism)</term><term>Interleukin-4 (metabolism)</term><term>Leukocytes, Mononuclear (immunology)</term><term>Leukocytes, Mononuclear (metabolism)</term><term>Microfilariae (metabolism)</term><term>Peptide Hydrolases (chemistry)</term><term>Peptide Hydrolases (pharmacology)</term><term>Th1 Cells (immunology)</term><term>Time Factors</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Agranulocytes (immunologie)</term><term>Agranulocytes (métabolisme)</term><term>Animaux</term><term>Anticorps antihelminthe ()</term><term>Anticorps antihelminthe (isolement et purification)</term><term>Antigènes d'helminthe ()</term><term>Antigènes d'helminthe (isolement et purification)</term><term>Brugia malayi (métabolisme)</term><term>Chromatographie d'affinité</term><term>Chromatographie en phase liquide</term><term>Cytokines (métabolisme)</term><term>Facteurs temps</term><term>Filariose lymphatique 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sur gel de polyacrylamide</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A FPLC purified 38kDa protease (Bm mf S-7) isolated from B. malayi microfilarial soluble antigen was identified. It showed pronounced reactivity with sera collected from 'putatively immune' asymptomatic and amicrofilaraemic individuals residing in an endemic area for bancroftian filariasis. Further the immune protective activity of Bm mf S-7 antigen was evaluated in susceptible hosts, jirds (Meriones unguiculatus) against B. malayi filarial infection. The antigen showed 89% cytotoxicity against mf and 87-89% against infective (L3) larvae in in vitro antibody dependent cellular cytotoxicity Assay (ADCC) and in situ micropore chamber methods. Bm mf S-7 immunized jirds after challenge infection showed 81.5% reduction in the adult worm burden. The present study has shown that, the 38kDa microfilarial proteases (Bm mf S-7) could stimulate a strong protective immune response against microfilariae and infective larvae in jird model to block the transmission of filariasis. Analysis of IgG subclasses against Bm mf S-7 revealed a significant increase in IgG2 and IgG3 antibodies in endemic normals. Lymphocyte proliferation to Bm mf S-7 was significantly high in endemic normal group as compared to that in clinical and microfilarial carriers. Significantly enhanced levels of IFN-gamma in the culture supernatant of PBMC of endemic normals followed by stimulation with Bm mf S-7 suggest that the cellular response in this group is skewed towards Th 1 type.</div></front></TEI><affiliations><list><country><li>Inde</li></country></list><tree><noCountry><name sortKey="Anandharaman, V" sort="Anandharaman, V" uniqKey="Anandharaman V" first="V" last="Anandharaman">V. Anandharaman</name><name sortKey="Dabir, Pankaj" sort="Dabir, Pankaj" uniqKey="Dabir P" first="Pankaj" last="Dabir">Pankaj Dabir</name><name sortKey="Kulkarni, Sandeep" sort="Kulkarni, Sandeep" uniqKey="Kulkarni S" first="Sandeep" last="Kulkarni">Sandeep Kulkarni</name><name sortKey="Reddy, M V R" sort="Reddy, M V R" uniqKey="Reddy M" first="M V R" last="Reddy">M V R. Reddy</name></noCountry><country name="Inde"><noRegion><name sortKey="Krithika, K N" sort="Krithika, K N" uniqKey="Krithika K" first="K N" last="Krithika">K N Krithika</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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